產品中心
華雅旗下紅榮微再授權代理(上海)TAKARA CellArtis,Rubicon產品 Cellartis-Y30055現貨數量有限 詳詢 1500 1904 520 紅榮微再-客服: 經銷商專員
iPS向肝臟細胞定向分化試劑盒
產品英文名稱:Cellartis® iPS Cell to Hepatocyte Differentiation System
產品中文名稱:iPS向肝臟細胞定向分化試劑盒
iPS向肝臟細胞定向分化各個步驟的培養(yǎng)
產品介紹
以肝臟細胞為模型的實驗越來越受到重視,在肝臟病變機理研究、藥物代謝研究、藥物毒理評估等方面有廣闊的應用前景。然而,以個體原代肝細胞為材料來源會產生如取材困難、材料批間差大、無法長期穩(wěn)定供應等一系列難以克服的問題。因此,通過iPS批量轉化生產肝細胞的方法就顯示出特別的優(yōu)勢。
Cellartis iPS Cell to Hepatocyte Differentiation System可以穩(wěn)定地、規(guī)?;貙崿FiPS向hepatocyte的誘導轉化生產,而且誘導轉化出的肝細胞能夠穩(wěn)定表達藥物代謝相關酶系統(tǒng)和藥物轉運系統(tǒng)。
Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系統(tǒng),客戶既可以對某個特殊病人來源的iPS實現轉化,也可以利用cellartis供應的iPS進行標準化操作。使用本產品的過程起始于誘導人iPS轉化成為確定性內胚層(Definitive Endoderm (DE))細胞,然后誘導轉化DE至肝細胞。本產品是All-In-One型產品,實驗所需的培養(yǎng)基、基質(coating)等包括在內。應用本產品轉化約3 × 106 hiPS cells,可以獲得約5 × 106 肝細胞(大約50 cm2面積的單層貼壁細胞)。
利用Cellartis iPS Cell to Hepatocyte Differentiation System轉化得到的肝細胞除了清晰表現出肝細胞的*特征外(如肝細胞特異性標志物、CYP系列酶等),還保留了原始細胞供體者的遺傳基因背景。另外,相比較從組織切除獲得的原代肝臟細胞(通常需要低溫保存),通過本產品系統(tǒng)轉化獲得的肝細胞在更長的時間跨度上保持著生理功能的穩(wěn)定性。這一點對于毒理學評估和病毒感染評估具有顯著的意義。
產品特點:
高度可重復的強大系統(tǒng) - *相同的方法已被證明可用于25種不同的iPS細胞系,因此無需針對您的細胞進行優(yōu)化。
理想的藥物代謝和安全性研究 - 始終如一地生成具有不同遺傳背景的> 90%純的功能性hiPS細胞來源的肝細胞。
定制的起始材料 - 從任何疾病相關的iPS細胞系開始,創(chuàng)建準確的肝臟疾病模型。
準備好個性化醫(yī)療 - 為治療應用制造針對患者的疾病特異性細胞。
擴展實驗窗口 - 肝細胞可用于功能測試至少11天。
Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.
Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.
Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.
Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.
Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.
產品應用:
iPS向肝臟細胞定向分化
組份:iPS細胞培養(yǎng)基+iPS分化內胚層培養(yǎng)基+內胚層分化肝細胞培養(yǎng)基
每種培養(yǎng)基都是*培養(yǎng)基
Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055):
Cellartis DEF-CS 100 Culture System (Cat. No. Y30020)
? 100 ml DEF-CS 100 Basal Medium
? 800 µl DEF-CS COAT-1 (for 100 ml)
? 300 µl DEF-CS GF-1 (for 100 ml)
? 100 µl DEF-CS GF-2 (for 100 ml)
? 40 µl DEF-CS GF-3 (for 100 ml)
Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030)
? 1 tube Definitive Endoderm Differentiation Coating (10 ml)
? 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)
? 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)
? 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)
? 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)
? 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)
? 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)
Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)
? 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)
? 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)
? 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)
? 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)
? 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)
? 1 tube Hepatocyte Coating (7.5 ml)
Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System詳詢
1500 1904 520 紅榮微再-客服: 經銷商專員
自備試劑:
人多能干細胞
胎牛血清(FBS)或血清替代品(KO-SR)
PBS Dulbecco's(含有Ca2 +和Mg2 +的D-PBS + / +)
PBS Dulbecco's不含Ca 2+和Mg 2+(D-PBS - / - )
TrypLE選擇酶(1X),不含酚紅
保存:
iPS細胞培養(yǎng)基添加劑–20°C,基礎培養(yǎng)基和包被劑2–8°C
iPS分化內胚層培養(yǎng)基和內胚層分化肝細胞培養(yǎng)基所有組份都保存在–20°C。
Cellartis DEF-CS 100 Culture System (Y30020):DEF-CS 100 Basal Medium ,COAT-1 2–8°C;GF-1, GF-2, and GF-3 –20°C
Cellartis Definitive Endoderm Differentiation Kit (Y30030):–20°C
Cellartis Hepatocyte Differentiation Kit (Y30050) :–20°C
產品性能:
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).
參考文獻:
Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance online publication. doi: 10.1007/s12015-015-9621-9
訂購信息
品牌 | 貨號 | 產品描述 | 規(guī)格 |
Cellartis | Y30051 | Cellartis® Hepatocyte Maintenance Medium | 1(50 mlx2) |
Cellartis | Y30055 | Cellartis® iPS Cell to Hepatocyte Differentiation System | 1 Kit |
肝細胞培養(yǎng)完整解決方案
人iPS誘導分化成肝細胞全過程的完整試劑盒:Y30055(新產品)
人iPS來源肝細胞株及*培養(yǎng)基Y10133/Y10134/Y10135(新產品)
人原代肝臟細胞培養(yǎng)基 Y20020(新產品)
http://www.chem17.com/st321032/article_1496885.html
華雅再生醫(yī)學旗艦公司:紅榮微再(上海)生物工程技術有限公司主營細胞治療和精準醫(yī)療等產品,2018年與TAKARA繼續(xù)合作,授權代理(上海)其旗下Cellartis干細胞研究制品和Rubicon單細胞文庫構建試劑。Cellartis公司位于瑞典,擁有iPS細胞等干細胞分化為肝細胞及臟器細胞的分化誘導技術和ES細胞,ips細胞,分化細胞等干細胞相關產品。
Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System 手機1500 1904 520 紅榮微再-客服 經銷商專員