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產(chǎn)品中心
PeproGrow™ EPC 內(nèi)皮祖細(xì)胞培養(yǎng)基
英文名:PeproGrow™ EPC (Endothelial Progenitor Cell Media)
中文名:PeproGrow 內(nèi)皮祖細(xì)胞培養(yǎng)基
PeproGrow EPC 適用培養(yǎng)內(nèi)皮祖細(xì)胞。能良好維持內(nèi)皮祖細(xì)胞的形態(tài)和功能,并提高內(nèi)皮一氧化氮合酶活性。
產(chǎn)品訂購(gòu)信息
品牌 | 貨號(hào) | 產(chǎn)品描述 | 包裝 |
PeproTech | 700-EPC | PeproGrow™ EPC (Endothelial Progenitor Cell Media)內(nèi)皮祖細(xì)胞培養(yǎng)基試劑盒 | 1 Kit |
產(chǎn)品描述
PeproTech提供三種內(nèi)皮細(xì)胞培養(yǎng)基,分別適用于體外培養(yǎng):骨髓或外周血來源的內(nèi)皮祖細(xì)胞(PeproGrow™ EPC ),大血管內(nèi)皮細(xì)胞(PeproGrow™ MacroV)和微血管內(nèi)皮細(xì)胞(PeproGrow™ MicroV)。這些培養(yǎng)基可以維持良好的內(nèi)皮細(xì)胞形態(tài)和功能,并能提高內(nèi)皮細(xì)胞關(guān)鍵的特異性標(biāo)志物——內(nèi)皮一氧化氮合酶(eNOS)的活性。由此,它們可為大血管、微血管內(nèi)皮細(xì)胞及EPCs提供優(yōu)良的細(xì)胞培養(yǎng)環(huán)境,且細(xì)胞生長(zhǎng)率高于目前市售的其他培養(yǎng)基。
Product Details
Catalog Number:700-EPC
Description:
PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro c*tion of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells.
PeproGrow EPC is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.
PeproGrow EPC適用
PeproGrow EPC is recommended for Endothelial Progenitor Cells:
PeproGrow EPC推薦內(nèi)皮祖細(xì)胞培養(yǎng):
Human Endothelial Progenitor Cells (hEPC) 人內(nèi)皮祖細(xì)胞
Mouse Endothelial Progenitor Cells (mEPC) 小鼠內(nèi)皮祖細(xì)胞
PeproGrow EPC特征
Figures 1 and 2: EPC Proliferation. EPC were seeded onto fibronectin-coated plates, and incubated for 7 days in PeproGrow EPC basal medium + Growth Supplement-EPC (PeproTech) and a competitor’s medium. The proliferation ability of EPC was assessed 7 days after cell c*tion using the XTT assay according to the manufacturer protocol. The proliferation ability of EPC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader for two independent assays run in triplicates.
圖一 EPC 擴(kuò)增:左 其他品牌;右 Peprotech
內(nèi)皮祖細(xì)胞擴(kuò)增。內(nèi)皮祖細(xì)胞接種在人纖連蛋白包被培養(yǎng)板上,分別使用PeproGrow EPC基礎(chǔ)培養(yǎng)基+EPC生長(zhǎng)因子(PeproTech)和其他品牌的培養(yǎng)基孵育7天。在細(xì)胞培養(yǎng)后7天用XTT分析培養(yǎng)基的內(nèi)皮祖細(xì)胞擴(kuò)增能力。
儲(chǔ)存:基礎(chǔ)培養(yǎng)基保存在2-8°C。生長(zhǎng)因子使用前保存在 -20°C。
內(nèi)皮一氧化氮合酶介紹
內(nèi)皮一氧化氮合酶是一種一氧化氮合成酶。其他還有神經(jīng)一氧化氮合酶和誘生性一氧化氮合酶。內(nèi)皮一氧化氮合酶主要作用于血管內(nèi)皮細(xì)胞的NO生成。NO對(duì)血管張力調(diào)節(jié),細(xì)胞擴(kuò)增,淋巴細(xì)胞粘連,血小板凝集都有重要作用。因此內(nèi)皮一氧化氮合酶功能是健康心血管系統(tǒng)的基礎(chǔ)。(英文:Wiki)
其他內(nèi)皮細(xì)胞培養(yǎng)基
ScienCell 1001 內(nèi)皮細(xì)胞培養(yǎng)基 現(xiàn)貨供應(yīng)(超鏈接)
PeproTech 內(nèi)皮細(xì)胞培養(yǎng)基系列
PeproTech提供三種內(nèi)皮細(xì)胞培養(yǎng)基試劑盒,分別適用于體外培養(yǎng):骨髓或外周血來源的內(nèi)皮祖細(xì)胞(PeproGrow™ EPC ),大血管內(nèi)皮細(xì)胞(PeproGrow™ MacroV)和微血管內(nèi)皮細(xì)胞(PeproGrow™ MicroV)。每款試劑盒由基礎(chǔ)培養(yǎng)基和一管單獨(dú)的內(nèi)皮細(xì)胞生長(zhǎng)添加劑組成。
品牌 | 貨號(hào) | 產(chǎn)品描述 | 包裝 |
PeproTech | 700-EPC | PeproGrow™ EPC (Endothelial Progenitor Cell Media)內(nèi)皮祖細(xì)胞培養(yǎng)基試劑盒 | 1Kit |
PeproTech | 700-MacroV | PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)大血管內(nèi)皮細(xì)胞培養(yǎng)基試劑盒 | 1Kit |
PeproTech | 700-MicroV | PeproGrow™ MicroV (Microvascular Endothelial Cell Media)微血管內(nèi)皮細(xì)胞培養(yǎng)基試劑盒 | 1Kit |
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